5a74_B : 3D-footprint complex

structure name	CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE I-CVUI IN COMPLEX WITH ITS TARGET (SRO1.3) IN THE PRESENCE OF 2 MM MN (DNA ENDONUCLEASE I-CVUI)
reference	LopezMendez et al. J.Biol.Chem. 290 28727 2015
source	CHLORELLA VULGARIS
experiment	X-ray (resolution=2.50, R-factor=0.191)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
multimeric complexes	5a72_AB 5a74_AB 5a77_AB 5a78_AB
redundant complexes	5a72_B 5a77_B 5a78_B
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 5a74_B interface=20,24,26,28,30,31,38,44,66,68,69,70,72,74,138, nfhdqlkfawlagfvdadgCinaQiVsReDYllkyqvRvsltvFqsttqhfilldiqkilgcgtvRkRNDgMsEfcvvggtslqttlekllpylqlkraqaklvlqiikklpntkdpsvlmeaalladkvglltdgkKrtilaenvreclkklghvvs
interface signature	CQVRDYRFRRNDMEK

Estimated binding specificities ?

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# IC=12.266 IC/col=1.022 n_of_columns=12

specificity:

A |   0  96   0  22  96   0   0   0  67  90  11  62
C |  96   0   0  16   0  96   0   0  28   1  11  11
G |   0   0  96  13   0   0  96   0   1   5   0   7
T |   0   0   0  45   0   0   0  96   0   0  74  16

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Dendrogram of similar interfaces ?

matrix format

  
  CA--QGVTRGDCYA--RT--FA--RG--RGNTDTMCEA------KT   L             +-5a74_B    
                                                       +---------1 
  STITQAKGRGSTNTQTSTATTC----YT--SC--VCDTRGSTKGTG   L   !         +-3mxb_B    
                                                     --3 
  SAIAQA----SC--RGKGQATAYC------ST--VA------KTTT   L   !   +-------2vbl_B    
                                                       +---2 
  SA--RARGRGKT----ECECKG------ST----ATRGRG------   L       +-------6uw0_B

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updated Tue Dec 19 09:55:01 2023