3mxa_A : 3D-footprint complex

structure name	MOLECULAR BASIS OF ENGINEERED MEGANUCLEASE TARGETING OF THE ENDOGENOUS HUMAN RAG1 LOCUS (scV3V2G19S)
reference	Subramanian et al. 'Nucleic 39 729 2011
source	Chlamydomonas reinhardtii
experiment	X-ray (resolution=2.30, R-factor=0.177)
structural superfamily	Homing endonucleases;
sequence family	LAGLIDADG endonuclease;
redundant complexes	3mx9_A
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 3mxa_A interface=21,23,25,31,37,39,43,45,65,67,69,72,74,139,169,171,173,175,177,179,180,185,187,191,193,215,217,220,222,224,226,286,287, ntkynkefllylagfvdgdgSiIaQinpnqSskfkhRlRltfYvTqktqrrwfldklvdeigvgYvRdSgsVsQyvlseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespdkflevctwvdqiaalndskTrkttsetvravlsnkefllylagfvdsdgSiIaQiKpRqSNkfkhQlSltfAvTqktqrrwfldklvdeigvgyvYdSgsVsDyRlSeikplhnfltqlqpflklkqkqanlvlkiieqlpsakespdkflevctwvdqiaalndsKTrkttsetvravlds
interface signature	SIQSRRYTYRSVQTSIQKRSNQSATYSVDRSKT

Estimated binding specificities ?

readout + contact

# IC=24.078 IC/col=1.094 n_of_columns=22

specificity:

A |   0   0   3   1   0  32   0  96  13  11   1  11   3   1   0   0  96   1   0   0  92  84
C |   0   0   1   1  96   0  96   0   7   7   1   6  86  88   0  96   0   1  96  96   3   6
G |   0  96   5   1   0   0   0   0  67  71   0   1   1   3   0   0   0  88   0   0   0   5
T |  96   0  87  93   0  64   0   0   9   7  94  78   6   4  96   0   0   6   0   0   1   1

scan!

Dendrogram of similar interfaces ?

matrix format

  
  STITQA------ST--RGRGYCTC----YCRG--SG--VAQT------TCSTITQAKG--RG--STNTQT--ATTC--YT--SC--VCDTRGSTKGTG   L                 +-3mxa_A    
                                                                                                                   +-----4 
  SAYCKAITRADCYG--------IG--------RT--DTIADG--IA----SAYCKTITPTRGDCYA--------IG----RT--DTICDG--IA----   L           !     +-3fd2_A    
                                                                                                           +-------5 
  SA--RARGRG--KT----ECECKG--------ST----ATRGRG------CAFTNT--LA--------QACTSA--LAKG------WCEA--TT----   L   !       !      +6uw0_B    
                                                                                                           !       +------2 
  SALTRGRGNT--KT--STECQGTAHAKA----ST--------KGTC----CAFCNT--SC--KG--TTQAKG--SCKGKG--KG--WCEA--TG----   L   !              +6uwj_A    
                                                                                                         --7 
  SAIAQA------SC--RGKGQATA----YC----ST--VA------KTTT------------------------------------------------   L   !                +2vbl_B    
                                                                                                           !              +-1 
  STITQAKGRG--STNTQTSTATTC------YT--SC--VCDTRGSTKGTG------------------------------------------------   L   !    +---------3 +3mxb_B    
                                                                                                           !    !         ! 
  CA--QGVTRG--DCYA--RT--FA------RGRGNTDTMCEA------KT------------------------------------------------   L   +----6         +5a74_B    
                                                                                                                ! 
  STITQANGNT--SC--RGRGYCTC------RG------VCQT------TT------------------------------------------------   L        +----------2xe0_A

home
updated Tue Dec 19 07:54:33 2023