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structure name	AN ASYMMETRIC COMPLEX OF RESTRICTION ENDONUCLEASE MSPI ON ITS PALINDROMIC DNA RECOGNITION SITE (TYPE II RESTRICTION ENZYME MSPI)
reference	Roberts et al. STRUCTURE 12 1741 2004
source	MORAXELLA SP.
experiment	X-ray (resolution=1.95, R-factor=0.225)
structural superfamily	Restriction endonuclease-like;
sequence family	Restriction endonuclease MspI;
redundant complexes	1yfi
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 1sa3_A interface=27,28,127,130,246,248,249,251,253,259,261, mrtellsklyddfgidqlphtqhgvtSDrlgklyekyildifkdieslkkyntnafpqekdisskllkalnldldniidvsssdtdlgrtiaggspktdatirftfhnqssrlvplnikhsskkkvSiaEydvetictgvgisdgelkelirkhqndqsaklftpvqkqrltellepyrerfirwcvtlraeksegnilhpdllirfqvidreyvdvtikniddyvsdriaegskarkpgfgtglNwTYaSgSkakkmQfKg
interface signature	SDSENTYSSQK

Estimated binding specificities ?

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# IC=3.283 IC/col=0.821 n_of_columns=4 specificity:     A | 0 0 32 10 C | 95 96 9 11 G | 0 0 48 66 T | 1 0 7 9 scan!

Related DNA sequences reported in the literature ?

site	source	matches (E-value)
term: MSPI
GGACCCGGGTCC	PubMed	1sa3_AB(4.00e-04), 1yfi_B(1.10e-02)
AGCCCGGGCT	PubMed	1sa3_A(9.07e-03), 1sa3_AB(4.53e-03), 1yfi_B(6.41e-03)
GGCCGG	PubMed	1sa3_A(9.83e-04), 1yfi_B(6.31e-04)
CCCGGG	PubMed	1sa3_A(9.83e-04), 1sa3_AB(1.66e-04), 1yfi_AB(2.38e-03), ... (total=4)

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updated Mon Dec 18 06:02:51 2023