3hqg_A : 3D-footprint complex

structure name	CRYSTAL STRUCTURE OF RESTRICTION ENDONUCLEASE ECORII CATALYTIC C- TERMINAL DOMAIN IN COMPLEX WITH COGNATE DNA (Type-2 restriction enzyme EcoRII)
reference	Tamulaitiene et al.
source	Escherichia coli
experiment	X-ray (resolution=2.60, R-factor=0.242)
structural superfamily	Restriction endonuclease-like;
sequence family	EcoRII C terminal;
links to other resources	NAKB PDIdb DNAproDB
protein sequence	> 3hqg_A interface=40,43,44,47,80,81,82,83,84,87,146,147,148, yilpedwhlrfpsgseiiqyaashyvknsldpdeqlldrRrvEYdiFllveelhvldiirkgfgsvdefialansvsnrRKSRAgkSlelhlehlfiehglrhfatqaitegnkkpdflfpsagayhdtefpvenlrmlavkttcKDRwrqilneadkihqvhlftlqegvslaqyremresgvrlvvpsslhkkypeavraelmtlgafiaeltglyadip
interface signature	REYFRKSRASKDR

Estimated binding specificities ?

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# IC=5.105 IC/col=1.021 n_of_columns=5

specificity:

A |  19   0  96   0   2
C |  38  96   0   0   2
G |  19   0   0  96  90
T |  20   0   0   0   2

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Related DNA sequences reported in the literature ?

site	source	matches (E-value)
term: EcoRII
CCNGG	PubMed	3hqg_A(1.35e-04)
CCAGG	PubMed	3hqg_A(2.56e-08)
CCTGG	PubMed	3hqg_A(2.56e-08)
CCATGG	PubMed	3hqg_A(2.63e-03)
CCTAGG	PubMed	3hqg_A(2.63e-03)
ACTGGG	PubMed	3hqg_A(3.56e-04)
CCNGGA	PubMed	3hqg_A(3.56e-04)
CCGGG	PubMed	3hqg_A(1.18e-03)

Dendrogram of similar interfaces ?

matrix format

  
  RAEAYAFA--------RAKCSCRAAASA----KGDGRG--   L            +------------------3hqg_A    
                                                   +------1 
  --------QGFCACKG--------------RGKGECVCRT   L   +-2      +------------------6eko_A    
                                                 ! ! 
  ----------------LT----------RT--EG------     --3 +-------------------------5gke_A    
                                                 ! 
  ------------------------------------ET--       +---------------------------6g3b_A

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updated Tue Dec 19 07:23:23 2023