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structure nameK38A ECORV BOUND TO CLEAVED DNA AND MN2+: P1 CRYSTAL FORM
(Type II restriction enzyme EcoRV)
referenceHorton et al. Biochemistry 43 6841 2004
sourceEscherichia coli
experimentX-ray (resolution=1.50, R-factor=?)
structural superfamilyRestriction endonuclease-like;
sequence familyRestriction endonuclease EcoRV;
multimeric complexes1az0_AB 1b94_AB 1b95_AB 1b96_AB 1b97_AB 1bgb_AB 1bss_AB 1bsu_AB 1bua_AB 1eo3_AB 1eo4_AB 1eon_AB 1eoo_AB 1eop_AB 1rv5_AB 1rva_AB 1rvb_AB 1rvc_AB 1stx_AB 1suz_AB 1sx5_AB 1sx8_AB 2b0d_AB 2b0e_AB 2ge5_AB 4rve_AB 5f8a_AB 5hlk_AB
redundant complexes1az0_A 1b94_A 1b95_B 1b96_A 1b97_B 1bgb_A 1bss_B 1bsu_B 1bua_A 1eo3_B 1eo4_B 1eon_A 1eoo_A 1eop_A 1rv5_A 1rva_B 1rvb_A 1rvc_A 1stx_A 1suz_A 1sx8_A 2b0d_B 2b0e_B 2ge5_A 4rve_C 5f8a_A 5hlk_B
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signatureNTSNTN

Estimated binding specificities ?

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# IC=3.142 IC/col=0.786 n_of_columns=4 specificity:   
A |  13  96  13  96
C |  13   0  16   0
G |  57   0  13   0
T |  13   0  54   0
scan!

Related DNA sequences reported in the literature ?


sitesourcematches (E-value)
term: ECORV
GATATCPubMed1sx5_A(3.59e-04), 1az0_AB(1.12e-03), 1b94_AB(1.18e-08), ... (total=41)
GAATTCPubMed1bsu_AB(1.12e-03), 1bua_AB(1.78e-03), 1eo3_AB(1.42e-07), ... (total=8)
GGGATATCCCPubMed1sx5_A(4.11e-03), 1b94_AB(6.71e-05), 1b95_AB(1.33e-05), ... (total=28)
AAAGATATCTTPubMed1sx5_A(5.65e-03), 1b94_AB(3.36e-07), 1b95_AB(2.70e-05), ... (total=28)
GATATPubMed1sx5_A(1.36e-04), 1az0_AB(3.54e-04), 1b94_AB(3.60e-06), ... (total=38)
GACGATATCGTCPubMed1sx5_A(7.21e-03), 1b94_AB(2.66e-04), 1b95_AB(4.80e-05), ... (total=28)
GTTATCPubMed1sx5_A(3.59e-04), 1b94_AB(3.29e-04), 1b95_AB(1.78e-04), ... (total=24)
GATCTCPubMed1b94_AB(3.29e-04), 1b95_AB(1.71e-04), 1b96_AB(1.71e-04), ... (total=24)
CGAGCTCGPubMed1bsu_AB(6.34e-03), 1eon_AB(6.34e-03), 2b0e_AB(6.34e-03)
CGGGATATCCCPubMed1sx5_A(5.65e-03), 1b94_AB(1.49e-04), 1b95_AB(2.70e-05), ... (total=27)
GATATGPubMed1sx5_A(3.59e-04), 1az0_AB(1.12e-03), 1b94_AB(3.89e-05), ... (total=38)
CACAACGATATCGGTTTGPubMed1b94_AB(1.63e-03), 1b95_AB(2.62e-04), 1b96_AB(2.62e-04), ... (total=17)
CCCATTTTGGGTTATCCCATTTTGGGPubMed1rv5_AB(6.58e-03), 1suz_AB(7.03e-03), 1sx8_AB(7.82e-03), ... (total=6)
GATATAPubMed1sx5_A(3.59e-04), 1az0_AB(1.12e-03), 1b94_AB(3.89e-05), ... (total=38)
AAAGATPubMed1b94_AB(1.07e-02), 1b96_B(8.85e-03), 1bss_B(4.79e-04), ... (total=4)
ATCTTPubMed1b94_AB(3.07e-03), 1b96_B(3.70e-03), 1bss_B(1.86e-04), ... (total=4)
GAGATCPubMed1b94_AB(3.29e-04), 1b95_AB(1.71e-04), 1b96_AB(1.71e-04), ... (total=24)
CAAACCGATATCGTTGTGPubMed1b94_AB(1.63e-03), 1b95_AB(2.62e-04), 1b96_AB(2.62e-04), ... (total=17)
TATATCPubMed1sx5_A(3.59e-04), 1az0_AB(1.12e-03), 1b94_AB(3.89e-05), ... (total=38)
CGAATTCGPubMed1bsu_AB(6.34e-03), 1bua_AB(9.21e-03), 1eo3_AB(3.82e-06), ... (total=8)
GCTATCPubMed1sx5_A(3.59e-04), 1b94_AB(3.29e-04), 1b95_AB(1.67e-04), ... (total=24)
CATATCPubMed1sx5_A(3.59e-04), 1az0_AB(1.12e-03), 1b94_AB(3.89e-05), ... (total=38)
CAGATGPubMed1b94_AB(1.07e-02), 1b96_B(8.85e-03), 1bss_B(4.79e-04), ... (total=4)
TGATAPubMed1sx5_A(1.36e-04), 1b94_AB(2.91e-03), 1b95_AB(1.77e-03), ... (total=23)
sitesourcematches (E-value)

Dendrogram of similar interfaces ?

matrix format
  
  --------NC----------TT--SGNATTNT   L                          +-1sx5_A    
                                            +-------------------1 
  --------NC----------TT--STNATTNT   L   +--2                   +-2ge5_B    
                                         !  ! 
  ------QCNT------QC--------------   L --3  +---------------------3ebc_A    
                                         ! 
  STEAKT----RCTTAT--NAKGDC--------   L   +------------------------3wvk_C

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updated Tue Dec 19 06:07:10 2023