Transcription Factor

Accessions: ECK120005684 (RegulonDB 7.5)
Names: MelR, MelR DNA-binding transcriptional dual regulator
Organisms: ECK12
Libraries: RegulonDB 7.5 1
1 Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muniz-Rascado L, Garcia-Sotelo JS, Weiss V, Solano-Lira H, Martinez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernandez S, Alquicira-Hernandez K, Lopez-Fuentes A, Porron-Sotelo L, Huerta AM, Bonavides-Martinez C, Balderas-Martinez YI, Pannier L, Olvera M, Labastida A, Jimenez-Jacinto V, Vega-Alvarado L, Del Moral-Chavez V, Hernandez-Alvarez A, Morett E, Collado-Vides J. RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more. Nucleic Acids Res. 2013 Jan 1;41(D1):D203-D213. [Pubmed]
Notes: carbon compounds; operon; activator; Transcription related; cytoplasm; transcription, DNA-dependent; sequence-specific DNA binding; intracellular; sequence-specific DNA binding transcription factor activity; DNA binding; regulation of transcription, DNA-dependent
Length: 303
Pfam Domains: 200-241 Bacterial regulatory helix-turn-helix proteins, AraC family
214-291 Helix-turn-helix domain
254-291 Bacterial regulatory helix-turn-helix proteins, AraC family
Sequence:
(in bold interface residues)
1 MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWHGQVEVNVPFDGDVEYL 60
61 INNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHLFLSWPLDKDLINHVTHGM 120
121 VIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLMLKRFSLSGWEPILVNKTSRT 180
181 HKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVKLNANYAMGIFQRVMQLTMKQYI 240
241 TAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTF 300
301 PG*
Interface Residues: 179, 183, 222, 223, 224, 225, 226, 227, 229, 230, 233, 271, 272, 275, 276, 280
3D-footprint Homologues: 1skn_P, 1xs9_A, 7vwz_G
Binding Motifs: MelR cwTTmCCwkAAAmtCAGA
Binding Sites: ECK120012349
ECK120013194
ECK120013740
ECK120013742
ECK120016174
Publications: Wade JT., Belyaeva TA., Hyde EI., Busby SJ. Repression of the Escherichia coli melR promoter by MelR: evidence that efficient repression requires the formation of a repression loop. Mol Microbiol. 36(1):223-9 (2000). [Pubmed]

Tamai E., Belyaeva TA., Busby SJ., Tsuchiya T. Mutations that increase the activity of the promoter of the Escherichia coli melibiose operon improve the binding of MelR, a transcription activator triggered by melibiose. J Biol Chem. 275(22):17058-63 (2000). [Pubmed]

Belyaeva TA., Wade JT., Webster CL., Howard VJ., Thomas MS., Hyde EI., Busby SJ. Transcription activation at the Escherichia coli melAB promoter: the role of MelR and the cyclic AMP receptor protein. Mol Microbiol. 36(1):211-22 (2000). [Pubmed]

Gallegos MT., Schleif R., Bairoch A., Hofmann K., Ramos JL. Arac/XylS family of transcriptional regulators. Microbiol Mol Biol Rev. 61(4):393-410 (1997). [Pubmed]

Wade JT., Belyaeva TA., Hyde EI., Busby SJ. A simple mechanism for co-dependence on two activators at an Escherichia coli promoter. EMBO J. 20(24):7160-7 (2001). [Pubmed]

Michan CM., Busby SJ., Hyde EI. The Escherichia coli MelR transcription activator: production of a stable fragment containing the DNA-binding domain. Nucleic Acids Res. 23(9):1518-23 (1995). [Pubmed]

Webster C., Gardner L., Busby S. The Escherichia coli melR gene encodes a DNA-binding protein with affinity for specific sequences located in the melibiose-operon regulatory region. Gene. 83(2):207-13 (1989). [Pubmed]

Caswell R., Williams J., Lyddiatt A., Busby S. Overexpression, purification and characterization of the Escherichia coli MelR transcription activator protein. Biochem J. 287 ( Pt 2):493-9 (1992). [Pubmed]

Bourgerie SJ., Michan CM., Thomas MS., Busby SJ., Hyde EI. DNA binding and DNA bending by the MelR transcription activator protein from Escherichia coli. Nucleic Acids Res. 25(9):1685-93 (1997). [Pubmed]

Grainger DC., Belyaeva TA., Lee DJ., Hyde EI., Busby SJ. Binding of the Escherichia coli MelR protein to the melAB promoter: orientation of MelR subunits and investigation of MelR-DNA contacts. Mol Microbiol. 48(2):335-48 (2003). [Pubmed]

Webster C., Gaston K., Busby S. Transcription from the Escherichia coli melR promoter is dependent on the cyclic AMP receptor protein. Gene. 68(2):297-305 (1988). [Pubmed]

Kahramanoglou C., Webster CL., El-Robh MS., Belyaeva TA., Busby SJ. Mutational analysis of the Escherichia coli melR gene suggests a two-state concerted model to explain transcriptional activation and repression in the melibiose operon. J Bacteriol. 188(9):3199-207 (2006). [Pubmed]

Tobes R., Ramos JL. AraC-XylS database: a family of positive transcriptional regulators in bacteria. Nucleic Acids Res. 30(1):318-21 (2002). [Pubmed]
Related annotations: PaperBLAST

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