Transcription Factor

Accessions: BHLHA15_DBD (HumanTF 1.0), BHLHA15_TF2 (HumanTF2 1.0), BHLHA15 (HT-SELEX2 May2017)
Names: BHA15_HUMAN, BHLHA15, bHLHb8, Class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, MIST-1, Muscle, intestine and stomach expression 1, ENSG00000180535
Organisms: Homo sapiens
Libraries: HumanTF 1.0 1, HumanTF2 1.0 2, HT-SELEX2 May2017 3
1 Jolma A, Yan J, Whitington T, Toivonen J, Nitta KR, Rastas P, Morgunova E, Enge M, Taipale M, Wei G, Palin K, Vaquerizas JM, Vincentelli R, Luscombe NM, Hughes TR, Lemaire P, Ukkonen E, Kivioja T, Taipale J. DNA-Binding Specificities of Human Transcription Factors. Cell. 2013 Jan 17;152(1-2):327-39. [Pubmed]
2 Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J. DNA-dependent formation of transcription factor pairs alters their binding specificity. Nature 527:384-8 (2015). [Pubmed]
3 Yin Y, Morgunova E, Jolma A, Kaasinen E, Sahu B, Khund-Sayeed S, Das PK, Kivioja T, Dave K, Zhong F, Nitta KR, Taipale M, Popov A, Ginno PA, Domcke S, Yan J, Schübeler D, Vinson C, Taipale J. Impact of cytosine methylation on DNA binding specificities of human transcription factors. Science : (2017). [Pubmed]
Uniprot: Q7RTS1
Notes: Ensembl ID: ENSG00000180535; DNA-binding domain sequence; TF family: bHLH; Clone source: Gene synthesis, Ensembl ID: ENSG00000180535; Construct type: TF2(3xFLAG); TF family: bHLH; Clone source: Jolma et al. 2013, TF family: bHLH experiment: HT-SELEX Hamming distance: 1 cycle: 2, TF family: bHLH experiment: Methyl-HT-SELEX Hamming distance: 1 cycle: 2
Length: 115
Pfam Domains: 28-79 Helix-loop-helix DNA-binding domain
Sequence:
(in bold interface residues)
1 AARAPGEGRRRRPGPSGPGGRRDSSIQRRLESNERERQRMHKLNNAFQALREVIPHVRAD 60
61 KKLSKIETLTLAKNYIKSLTATILTMSSSRLPGLEGPGPKLYQHYQQQQQVAGGA
Interface Residues: 29, 31, 32, 33, 35, 36, 37, 39, 40, 63, 65, 87
3D-footprint Homologues: 2ypa_A, 7z5k_B, 1an4_A, 7ssa_L, 5eyo_A, 6od3_F, 5i50_B, 2ypa_B, 2ql2_A, 2ql2_D, 4h10_A, 5nj8_D, 5v0l_A, 5gnj_I, 7xhv_B, 7xi3_B, 3zdb_A
Binding Motifs: BHLHA15_DBD ACCATATGkt
ARNTL_BHLHA15 rtCACGTGMacmCATATGgk
CLOCK_BHLHA15 wCAYGTGkcyarCAyrTGs
ETV2_BHLHA15 rsCGGAAmCATATGk
FLI1_BHLHA15 wcCGGAAACATATGk
RFX3_BHLHA15 TrGCaACgavCATATGkt
BHLHA15_3 rmCATATGky
BHLHA15_4 avCAGCTGyy
BHLHA15_methyl_1 rmCATATGky
BHLHA15_methyl_2 amCAGCTGkt
Publications: Jolma A, Yan J, Whitington T, Toivonen J, Nitta KR, Rastas P, Morgunova E, Enge M, Taipale M, Wei G, Palin K, Vaquerizas JM, Vincentelli R, Luscombe NM, Hughes TR, Lemaire P, Ukkonen E, Kivioja T, Taipale J. DNA-Binding Specificities of Human Transcription Factors. Cell. 2013 Jan 17;152(1-2):327-39. [Pubmed]

Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J. DNA-dependent formation of transcription factor pairs alters their binding specificity. Nature 527:384-8 (2015). [Pubmed]
Related annotations: PaperBLAST

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These data are available AS IS and at your own risk. The EEAD/CSIC do not give any representation or warranty nor assume any liability or responsibility for the data nor the results posted (whether as to their accuracy, completeness, quality or otherwise). Access to these data is available free of charge for ordinary use in the course of research. Downloaded data have CC-BY-NC-SA license. FootprintDB is also available at RSAT::Plants, part of the INB/ELIXIR-ES resources portfolio.