Transcription Factor
| Accessions: | T161918_1.02 (CISBP 1.02), UP00322A (UniPROBE 20160601), P04386 (JASPAR 2024) |
| Names: | GAL4, T161918_1.02;, Regulatory protein GAL4, YPL248C, GAL4_YEAST |
| Organisms: | Saccharomyces cerevisiae |
| Libraries: | CISBP 1.02 1, UniPROBE 20160601 2, JASPAR 2024 3 1 Weirauch MT, Yang A, Albu M, Cote AG, Montenegro-Montero A, Drewe P, Najafabadi HS, Lambert SA, Mann I, Cook K, Zheng H, Goity A, van Bakel H, Lozano JC, Galli M, Lewsey MG, Huang E, Mukherjee T, Chen X, Reece-Hoyes JS, Govindarajan S, Shaulsky G, et al. Determination and inference of eukaryotic transcription factor sequence specificity. Cell. 2014 Sep 11;158(6):1431-43. doi: 10.1016/j.cell.2014.08.009. [Pubmed] 2 Hume MA, Barrera LA, Gisselbrecht SS, Bulyk ML. UniPROBE, update 2015: new tools and content for the online database of protein-binding microarray data on protein-DNA interactions. Nucleic Acids Res : (2015). [Pubmed] 3 Rauluseviciute I, Riudavets-Puig R, Blanc-Mathieu R, Castro-Mondragon JA, Ferenc K, Kumar V, Lemma RB, Lucas J, Cheneby J, Baranasic D, Khan A, Fornes O, Gundersen S, Johansen M, Hovig E, Lenhard B, Sandelin A, Wasserman WW, Parcy F, Mathelier A. JASPAR 2024: 20th anniversary of the open-access database of transcription factor binding profiles. Nucleic Acids Res : (2023). [Pubmed] |
| Description: | DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p |
| Notes: | experiment type:PBM, family:Zinc cluster |
| Length: | 881 |
| Pfam Domains: | 9-47 Fungal Zn(2)-Cys(6) binuclear cluster domain 50-106 Gal4-like dimerisation domain 244-469 Fungal specific transcription factor domain |
| Sequence: (in bold interface residues) | 1 MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESR 60 61 LERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPL 120 121 TLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDFMPRDALHGFDWSEEDDM 180 181 SDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVNRLPTMITDRYTLASRSTT 240 241 SRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQILFNCILAIGAWCIEGESTD 300 301 IDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQKTNTSYNFHSFSIRMAISLG 360 361 LNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRSIQLSQNTISFPSSVDDVQRTT 420 421 TGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICAKKCLMICNEIEEVSRQAPKFLQ 480 481 MDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVLRDFFTNFTQKKSQLEQDQNDHQS 540 541 YEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAWNCSYYLFNAVLVPIKTLLSNSKSN 600 601 AENNETAQLLQQINTVLMLLKKLATFKIQTCEKYIQVLEEVCAPFLLSQCAIPLPHISYN 660 661 NSNGSAIKNIVGSATIAQYPTLPEENVNNISVKYVSPGSVGPSPVPLKSGASFSDLVKLL 720 721 SNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQLQSLVPLTPSALFGGANFNQSGNIADSS 780 781 LSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGW 840 841 TDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE |
| Interface Residues: | 6, 17, 18, 19, 45, 46, 47, 48 |
| 3D-footprint Homologues: | 6o19_A, 1hwt_C, 1pyi_A, 2er8_C, 3coq_A, 1zme_D, 7uik_T, 1d66_B |
| Binding Motifs: | MA0299.1 CGGrsvAsagtrytC UP00322A_1 yCGGgnnnnnnnnnnCCGr M1832_1.02 cyCCGrr |
| Binding Sites: | AACTCCGA ACTCCGAA ACTCCGGA AGCTCCGA AGCTCCGG CCCCCGAA CCTCCGAA CCTCCGGA CTCCGAAA CTCCGAAG CTCCGAGA CTCCGGAC CTCCGGAG CTCGGAAC GCTCCGGA GTCCGGAC TCCGGAGA TTCCGGAA CCCCGGAG CCTCCGAG |
| Publications: | *Zhu C, *Byers K, *McCord RP, Shi Z, Berger MF, Newburger D, Saulrieta K, Smith Z, Shah M, Radhakrishnan M, Philippakis AA, Hu Y, De Masi F, Pacek M, Rolfs A , Murthy TVS, LaBaer J, Bulyk ML. High-Resolution DNA Binding Specificity Analysis of Yeast Transcription Factors. Genome Research. Epub January 21, 2009, doi:10.1101/gr.090233.108 (*co-1st authors). [Pubmed] Newburger D.E, Bulyk M.L. UniPROBE: an online database of protein binding microarray data on protein-DNA interactions. Nucleic acids research 37:D77-82 (2009). [Pubmed] |
| Related annotations: | PaperBLAST |
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