Transcription Factor

Accessions: ECK120005000 (RegulonDB 7.5)
Names: LeuO, LeuO DNA-binding transcriptional dual activator
Organisms: ECK12
Libraries: RegulonDB 7.5 1
1 Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muniz-Rascado L, Garcia-Sotelo JS, Weiss V, Solano-Lira H, Martinez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernandez S, Alquicira-Hernandez K, Lopez-Fuentes A, Porron-Sotelo L, Huerta AM, Bonavides-Martinez C, Balderas-Martinez YI, Pannier L, Olvera M, Labastida A, Jimenez-Jacinto V, Vega-Alvarado L, Del Moral-Chavez V, Hernandez-Alvarez A, Morett E, Collado-Vides J. RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more. Nucleic Acids Res. 2013 Jan 1;41(D1):D203-D213. [Pubmed]
Notes: LeuO is a dual transcriptional regulator that regulates genes involved in leucine biosynthesis Chen CC,2005; Hertzberg KM,1980, genes involved in the utilization of certain β-glucosides Ueguchi C,1998; Madhusudan S,2005 and genes encoding LuxR-type transcription factors Stratmann T,2008 It is also involved in the bacterial stringent response Majumder A,2001.LeuO is one of the transcription factors that counteract H-NS-mediated repression of specific loci Chen CC,2005; Madhusudan S,2005; Ueguchi C,1998 Overproduction of LeuO causes the phenotype Bgl+, since LeuO can unsilence the bglGFB operon, which is silenced (phenotypically Bgl ) under laboratory conditions Ueguchi C,1998LeuO is part of the RpoS/H-NS/Hfq/LeuO/DsrA RNA regulatory cascade that controls the bglGFH operon Madhusudan S,2005and translation of rpoS, particularly at low temperatures Klauck E,1997; Repoila F,2003.LeuO belongs to the LysR transcriptional regulator family and contains a helix-turn-helix DNA-binding domain Ueguchi C, 1998; Henikoff S,1988 No LeuO consensus binding sequence is known Stratmann T,2008.LeuO activates transcription of the divergent leuLABCD operon Chen CC,2001.An in vivo genetic selection (SELEX) and phenotype microarray analysis revealed several multidrug resistance genes as targets for LeuO, including acrEF, ygcLKJIH-ygbTF, and mdtNOP (sdsRQP); mdtNOP (sdsRQP) is involved in sensitivity control against sulfa drugs Shimada T,2009; activator; regulation of transcription, DNA-dependent; sequence-specific DNA binding transcription factor activity; DNA binding; transcription, DNA-dependent; leucine; operon; cytoplasm; Transcription related
Length: 315
Pfam Domains: 24-81 Bacterial regulatory helix-turn-helix protein, lysR family
138-309 LysR substrate binding domain
Sequence:
(in bold interface residues)
1 MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARL 60
61 KVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCS 120
121 PLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQETEFVISYEDFHRPEFTSVP 180
181 LFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG 240
241 MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKQNSRTCYLSWHEAAGRDKG 300
301 HQWMEEQLVSICKR*
Interface Residues: 49, 50, 51, 52, 55, 74, 233, 234, 237, 238
3D-footprint Homologues: 4iht_D, 8gkh_P
Binding Motifs: LeuO WTThdyRGKRdWTrTkAT
Binding Sites: ECK125109446
ECK125110253
ECK125110255
ECK125141924
ECK125141926
ECK125141928
ECK125141930
ECK125141932
ECK125141934
ECK125141936
Publications: Chen CC., Chou MY., Huang CH., Majumder A., Wu HY. A cis-spreading nucleoprotein filament is responsible for the gene silencing activity found in the promoter relay mechanism. J Biol Chem. 280(6):5101-12 (2005). [Pubmed]

Hertzberg KM., Gemmill R., Jones J., Calvo JM. Cloning of an EcoRI-generated fragment of the leucine operon of Salmonella typhimurium. Gene. 8(2):135-52 (1980). [Pubmed]

Ueguchi C., Ohta T., Seto C., Suzuki T., Mizuno T. The leuO gene product has a latent ability to relieve bgl silencing in Escherichia coli. J Bacteriol. 180(1):190-3 (1998). [Pubmed]

Madhusudan S., Paukner A., Klingen Y., Schnetz K. Independent regulation of H-NS-mediated silencing of the bgl operon at two levels: upstream by BglJ and LeuO and downstream by DnaKJ. Microbiology. 151(Pt 10):3349-59 (2005). [Pubmed]

Stratmann T., Madhusudan S., Schnetz K. Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO. J Bacteriol. 190(3):926-35 (2008). [Pubmed]

Chen CC., Fang M., Majumder A., Wu HY. A 72-base pair AT-rich DNA sequence element functions as a bacterial gene silencer. J Biol Chem. 276(12):9478-85 (2001). [Pubmed]

Klauck E., Bohringer J., Hengge-Aronis R. The LysR-like regulator LeuO in Escherichia coli is involved in the translational regulation of rpoS by affecting the expression of the small regulatory DsrA-RNA. Mol Microbiol. 25(3):559-69 (1997). [Pubmed]

Repoila F., Gottesman S. Temperature sensing by the dsrA promoter. J Bacteriol. 185(22):6609-14 (2003). [Pubmed]

Henikoff S., Haughn GW., Calvo JM., Wallace JC. A large family of bacterial activator proteins. Proc Natl Acad Sci U S A. 85(18):6602-6 (1988). [Pubmed]

Shimada T., Yamamoto K., Ishihama A. Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux. J Bacteriol. 191(14):4562-71 (2009). [Pubmed]

Majumder A., Fang M., Tsai KJ., Ueguchi C., Mizuno T., Wu HY. LeuO expression in response to starvation for branched-chain amino acids. J Biol Chem. 276(22):19046-51 (2001). [Pubmed]
Related annotations: PaperBLAST

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