Transcription Factor

Accessions: E2F8_DBD (HumanTF 1.0), E2F8_TF2 (HumanTF2 1.0), E2F8 (HT-SELEX2 May2017)
Names: E2F-8, E2F8, E2F8_HUMAN, Transcription factor E2F8, ENSG00000129173
Organisms: Homo sapiens
Libraries: HumanTF 1.0 1, HumanTF2 1.0 2, HT-SELEX2 May2017 3
1 Jolma A, Yan J, Whitington T, Toivonen J, Nitta KR, Rastas P, Morgunova E, Enge M, Taipale M, Wei G, Palin K, Vaquerizas JM, Vincentelli R, Luscombe NM, Hughes TR, Lemaire P, Ukkonen E, Kivioja T, Taipale J. DNA-Binding Specificities of Human Transcription Factors. Cell. 2013 Jan 17;152(1-2):327-39. [Pubmed]
2 Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J. DNA-dependent formation of transcription factor pairs alters their binding specificity. Nature 527:384-8 (2015). [Pubmed]
3 Yin Y, Morgunova E, Jolma A, Kaasinen E, Sahu B, Khund-Sayeed S, Das PK, Kivioja T, Dave K, Zhong F, Nitta KR, Taipale M, Popov A, Ginno PA, Domcke S, Yan J, Schübeler D, Vinson C, Taipale J. Impact of cytosine methylation on DNA binding specificities of human transcription factors. Science : (2017). [Pubmed]
Uniprot: A0AVK6
Notes: Ensembl ID: ENSG00000129173; DNA-binding domain sequence; TF family: E2F; Clone source: Gene synthesis, Ensembl ID: ENSG00000129173; Construct type: TF2(3xFLAG); TF family: E2F; Clone source: Jolma et al. 2013, TF family: E2F experiment: HT-SELEX Hamming distance: 1 cycle: 2, TF family: E2F experiment: Methyl-HT-SELEX Hamming distance: 1 cycle: 2
Length: 262
Pfam Domains: 14-83 E2F/DP family winged-helix DNA-binding domain
162-248 E2F/DP family winged-helix DNA-binding domain
Sequence:
(in bold interface residues)
1 HLSGDEFEKSQPSRKEKSLGLLCHKFLARYPNYPNPAVNNDICLDEVAEELNVERRRIYD 60
61 IVNVLESLHMVSRLAKNRYTWHGRHNLNKTLGTLKSIGEENKYAEQIMMIKKKEYEQEFD 120
121 FIKSYSIEDHIIKSNTGPNGHPDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVMLFLV 180
181 STPQIVSLEVAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGR 240
241 KPAFKWTGPEISPNTSGSSPVI
Interface Residues: 11, 12, 14, 52, 54, 55, 56, 57, 59, 211, 214, 215, 217
3D-footprint Homologues: 6p7b_B, 1cf7_A, 4yo2_A, 1cf7_B
Binding Motifs: E2F8_DBD TTTCCCGCCAAA
GCM2_E2F8 ATrsGGGtwatsGcGGGAaw
TFAP2C_E2F8 ttTCCCGCbmsCCcsmGGs
E2F8_2 wtTTCCCGCCAaaw
E2F8_methyl_1 wtTTCCCGCcAAaw
Publications: Jolma A, Yan J, Whitington T, Toivonen J, Nitta KR, Rastas P, Morgunova E, Enge M, Taipale M, Wei G, Palin K, Vaquerizas JM, Vincentelli R, Luscombe NM, Hughes TR, Lemaire P, Ukkonen E, Kivioja T, Taipale J. DNA-Binding Specificities of Human Transcription Factors. Cell. 2013 Jan 17;152(1-2):327-39. [Pubmed]

Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J. DNA-dependent formation of transcription factor pairs alters their binding specificity. Nature 527:384-8 (2015). [Pubmed]
Related annotations: PaperBLAST

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