Transcription Factor

Accessions: UP00382A (UniPROBE 20160601)
Names: MDL-1
Organisms: Caenorhabditis elegans
Libraries: UniPROBE 20160601 1
1 Hume MA, Barrera LA, Gisselbrecht SS, Bulyk ML. UniPROBE, update 2015: new tools and content for the online database of protein-binding microarray data on protein-DNA interactions. Nucleic Acids Res : (2015). [Pubmed]
Uniprot: Q21663
Description: MAD-Like: mdl-1 encodes a basic helix-loop-helix (bHLH) protein similar to the vertebrate MAD transcriptional regulators; in vitro, MDL-1 can heterodimerize, and bind an E-box DNA sequence, with MXL-1, a C. elegans MAX-like bHLH protein; when expressed in rat embryonic fibroblasts, MDL-1 is able to suppress c-MYC/RAS-induced cell transformation, in a manner dependent upon an intact, predicted SIN3 interaction domain; mdl-1::gfp promoter fusions are expressed in a number of different tissues, including the
Length: 280
Pfam Domains: 96-147 Helix-loop-helix DNA-binding domain
Sequence:
(in bold interface residues)
1 EQQLNLGHLLTAARLLDIGALDISSLDLGALTSTSSSPGSSSPAMFELSNESELRSLFCG 60
61 KLKVDKKQSSCASNASTSSQPYCSSPPARKSSKHSRTAHNELEKTRRANLRGCLETLKML 120
121 VPCVSDATRNTTLALLTRARDHIIELQDSNAAQMKKLNDLRDEQDELVAELAQLQADEEV 180
181 AQATSQACQTLSQSRPESRASSFTSTSSRDSPCYLEYSPSSKPMDSHKPTIIDLYAEGLI 240
241 PRGPITFPRPLVYPHNVFDLMNLPPTPFDVSQFLPINLQV
Interface Residues: 98, 99, 100, 102, 103, 106, 107, 126, 129, 134, 138
3D-footprint Homologues: 1an4_A, 7f2f_B, 4h10_A, 5v0l_A, 1am9_A, 7ssa_L, 5eyo_A, 6g1l_A, 7xi3_A, 5i50_B, 5nj8_D, 2ql2_D, 8osl_P, 6ny1_Y
Binding Motifs: UP00382A_1 Awkwwtwaaaawwa
UP00382A_2 twdasCACGTGskawb
Binding Sites: AACACGTG
ACACGTGC
ACACGTGG
ACCACGTG
AGCACGTG
ATCACGTG
CACGTGAC
CACGTGCA
CACGTGCC
CACGTGCG
CACGTGGA
CACGTGGC
CACGTGGG
CACGTGTC
CCACGTGA
CCACGTGC
CCACGTGG
GCACGTGA
GCACGTGC
TCACGTGA
Publications: Grove C.A, De Masi F, Barrasa M.I, Newburger D.E, Alkema M.J, Bulyk M.L, Walhout A.J. A multiparameter network reveals extensive divergence between C. elegans bHLH transcription factors. Cell 138:314-27 (2009). [Pubmed]
Related annotations: PaperBLAST

Disclaimer and license

These data are available AS IS and at your own risk. The EEAD/CSIC do not give any representation or warranty nor assume any liability or responsibility for the data nor the results posted (whether as to their accuracy, completeness, quality or otherwise). Access to these data is available free of charge for ordinary use in the course of research. Downloaded data have CC-BY-NC-SA license. FootprintDB is also available at RSAT::Plants, part of the INB/ELIXIR-ES resources portfolio.