Transcription Factor

Accessions: T154048_1.02 (CISBP 1.02), Q9FLX8 (JASPAR 2024), T21742 (AthalianaCistrome v4_May2016)
Names: T154048_1.02;, WRKY27, Probable WRKY transcription factor 27, WRK27_ARATH, WRKY DNA-binding protein 27, AT5G52830, T21742;
Organisms: Arabidopsis thaliana
Libraries: CISBP 1.02 1, JASPAR 2024 2, AthalianaCistrome v4_May2016 3
1 Weirauch MT, Yang A, Albu M, Cote AG, Montenegro-Montero A, Drewe P, Najafabadi HS, Lambert SA, Mann I, Cook K, Zheng H, Goity A, van Bakel H, Lozano JC, Galli M, Lewsey MG, Huang E, Mukherjee T, Chen X, Reece-Hoyes JS, Govindarajan S, Shaulsky G, et al. Determination and inference of eukaryotic transcription factor sequence specificity. Cell. 2014 Sep 11;158(6):1431-43. doi: 10.1016/j.cell.2014.08.009. [Pubmed]
2 Rauluseviciute I, Riudavets-Puig R, Blanc-Mathieu R, Castro-Mondragon JA, Ferenc K, Kumar V, Lemma RB, Lucas J, Cheneby J, Baranasic D, Khan A, Fornes O, Gundersen S, Johansen M, Hovig E, Lenhard B, Sandelin A, Wasserman WW, Parcy F, Mathelier A. JASPAR 2024: 20th anniversary of the open-access database of transcription factor binding profiles. Nucleic Acids Res : (2023). [Pubmed]
3 O'Malley RC, Huang SS, Song L, Lewsey MG, Bartlett A, Nery JR, Galli M, Gallavotti A, Ecker JR. Cistrome and Epicistrome Features Shape the Regulatory DNA Landscape. Cell 165:1280-92 (2016). [Pubmed]
Notes: experiment type:PBM, family:WRKY, ecotype:Col-0, experiment type: ampDAP-seq, experiment type: ampDAP-seq (methyl-cytosines removed by PCR), experiment type: DAP-seq
Length: 348
Pfam Domains: 165-223 WRKY DNA -binding domain
Sequence:
(in bold interface residues)
1 MSSEDWDLFAVVRSCSSSVSTTNSCAGHEDDIGNCKQQQDPPPPPLFQASSSCNELQDSC 60
61 KPFLPVTTTTTTTWSPPPLLPPPKASSPSPNILLKQEQVLLESQDQKPPLSVRVFPPSTS 120
121 SSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTICHVTQENLSSDLWAWRKYGQKPIKG 180
181 SPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNKSQ 240
241 PVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNGDEDMVGQEVNMEEEEEEEEV 300
301 EEDDEEEEDDDDVDDLLIPNLAVRDRDDLFFAGSFPSWSAGSAGDGGG
Interface Residues: 171, 172, 173, 175, 176, 187
3D-footprint Homologues: 6j4e_B, 6j4f_F, 6j4g_B, 6ir8_A, 7z0u_A
Binding Motifs: M1700_1.02 drGTCaamg
M0822 / MA1318.1 akmGTTGACTTTt
M0793 aAAAGTCAACkwt
MA1318.2 mGTTGACTTTt
Binding Sites: MA1318.1.19
MA1318.1.15
MA1318.1.14
MA1318.1.4
MA1318.1.20
MA1318.1.18
MA1318.1.11
MA1318.1.12
MA1318.1.5
MA1318.1.2 / MA1318.1.7
MA1318.1.13
MA1318.1.8
MA1318.1.16 / MA1318.1.17
MA1318.1.6
MA1318.1.1
MA1318.1.10
MA1318.1.3
MA1318.1.9
MA1318.2.13
MA1318.2.14
MA1318.2.9
MA1318.2.18
MA1318.2.5
MA1318.2.8
MA1318.2.6
MA1318.2.1
MA1318.2.10
MA1318.2.11
MA1318.2.12
MA1318.2.15
MA1318.2.16
MA1318.2.17
MA1318.2.19
MA1318.2.2 / MA1318.2.7
MA1318.2.20
MA1318.2.3
MA1318.2.4
Publications: Yu D, Chen C, Chen Z. 2001. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell. 13:1527-40. [Pubmed]
Related annotations: PaperBLAST

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