Transcription Factor

Accessions: ECK120008762 (RegulonDB 7.5)
Names: DcuR, DcuR transcriptional activator
Organisms: ECK12
Libraries: RegulonDB 7.5 1
1 Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muniz-Rascado L, Garcia-Sotelo JS, Weiss V, Solano-Lira H, Martinez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernandez S, Alquicira-Hernandez K, Lopez-Fuentes A, Porron-Sotelo L, Huerta AM, Bonavides-Martinez C, Balderas-Martinez YI, Pannier L, Olvera M, Labastida A, Jimenez-Jacinto V, Vega-Alvarado L, Del Moral-Chavez V, Hernandez-Alvarez A, Morett E, Collado-Vides J. RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more. Nucleic Acids Res. 2013 Jan 1;41(D1):D203-D213. [Pubmed]
Notes: two-component signal transduction system (phosphorelay); two-component response regulator activity; transcription, DNA-dependent; transcription activator activity; DNA binding; Transcription related; regulation of transcription, DNA-dependent; cytoplasm; activator; two component regulatory systems (external signal); anaerobic respiration; intracellular signal transduction; response to external stimulus; nucleic acid binding transcription factor activity
Length: 240
Pfam Domains: 4-117 Response regulator receiver domain
Sequence:
(in bold interface residues)
1 MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQ 60
61 KENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGW 120
121 RQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLTPQTLRTLCQWIDAHQDYEFS 180
181 TDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYCQ* 240
Interface Residues: 79, 181, 182, 186, 191, 192, 193, 194, 196, 197, 201, 230, 231, 235
3D-footprint Homologues: 5v07_Z, 4wwc_B, 4u0y_B, 3vk8_B, 7vwz_G
Binding Motifs: DcuR TkwwTTwAaTTwykwwA
Binding Sites: ECK120011350
ECK120011352
ECK120011976
ECK120035052
ECK125141266
ECK125141268
Publications: Oshima T., Aiba H., Masuda Y., Kanaya S., Sugiura M., Wanner BL., Mori H., Mizuno T. Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12. Mol Microbiol. 46(1):281-91 (2002). [Pubmed]

Hirakawa H., Nishino K., Hirata T., Yamaguchi A. Comprehensive studies of drug resistance mediated by overexpression of response regulators of two-component signal transduction systems in Escherichia coli. J Bacteriol. 185(6):1851-6 (2003). [Pubmed]

Abo-Amer AE., Munn J., Jackson K., Aktas M., Golby P., Kelly DJ., Andrews SC. DNA interaction and phosphotransfer of the C4-dicarboxylate-responsive DcuS-DcuR two-component regulatory system from Escherichia coli. J Bacteriol. 186(6):1879-89 (2004). [Pubmed]

Janausch IG., Garcia-Moreno I., Unden G. Function of DcuS from Escherichia coli as a fumarate-stimulated histidine protein kinase in vitro. J Biol Chem. 277(42):39809-14 (2002). [Pubmed]

Janausch IG., Garcia-Moreno I., Lehnen D., Zeuner Y., Unden G. Phosphorylation and DNA binding of the regulator DcuR of the fumarate-responsive two-component system DcuSR of Escherichia coli. Microbiology. 150(Pt 4):877-83 (2004). [Pubmed]

Oyamada T., Yokoyama K., Morinaga M., Suzuki M., Makino K. Expression of Escherichia coli DcuS-R two-component regulatory system is regulated by the secondary internal promoter which is activated by CRP-cAMP. J Microbiol. 45(3):234-40 (2007). [Pubmed]

Goh EB., Bledsoe PJ., Chen LL., Gyaneshwar P., Stewart V., Igo MM. Hierarchical control of anaerobic gene expression in Escherichia coli K-12: the nitrate-responsive NarX-NarL regulatory system represses synthesis of the fumarate-responsive DcuS-DcuR regulatory system. J Bacteriol. 187(14):4890-9 (2005). [Pubmed]

Golby P., Davies S., Kelly DJ., Guest JR., Andrews SC. Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-dicarboxylates in Escherichia coli. J Bacteriol. 181(4):1238-48 (1999). [Pubmed]

Zientz E., Bongaerts J., Unden G. Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system. J Bacteriol. 180(20):5421-5 (1998). [Pubmed]
Related annotations: PaperBLAST

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