Transcription Factor

Accessions: ECK120004565 (RegulonDB 7.5)
Names: AsnC
Organisms: ECK12
Libraries: RegulonDB 7.5 1
1 Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muniz-Rascado L, Garcia-Sotelo JS, Weiss V, Solano-Lira H, Martinez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernandez S, Alquicira-Hernandez K, Lopez-Fuentes A, Porron-Sotelo L, Huerta AM, Bonavides-Martinez C, Balderas-Martinez YI, Pannier L, Olvera M, Labastida A, Jimenez-Jacinto V, Vega-Alvarado L, Del Moral-Chavez V, Hernandez-Alvarez A, Morett E, Collado-Vides J. RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more. Nucleic Acids Res. 2013 Jan 1;41(D1):D203-D213. [Pubmed]
Notes: cytoplasm; regulon; positive regulation of transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; response to amino acid stimulus; amino acid binding; sequence-specific DNA binding; intracellular; sequence-specific DNA binding transcription factor activity; transcription activator activity; asparagine; Transcription related; activator; repressor; specific transcriptional repressor activity
Length: 153
Pfam Domains: 6-47 AsnC-type helix-turn-helix domain
6-53 Winged helix-turn-helix DNA-binding
24-66 HTH domain
75-143 AsnC family
Sequence:
(in bold interface residues)
1 MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVEKMKQAGIITGARIDVS 60
61 PKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTTGHYSIFIKVMCRSIDALQ 120
121 HVLINKIQTIDEIQSTETLIVLQNPIMRTIKP*
Interface Residues: 25, 35, 36, 37, 38, 40, 45, 62
3D-footprint Homologues: 2e1c_A, 5haw_A, 1z9c_A
Binding Motifs: AsnC KKWTTrwTGMwT
Binding Sites: ECK120048773
ECK120048775
ECK120048792
ECK120048794
Publications: Kolling R., Lother H. AsnC: an autogenously regulated activator of asparagine synthetase A transcription in Escherichia coli. J Bacteriol. 164(1):310-5 (1985). [Pubmed]

de Wind N., de Jong M., Meijer M., Stuitje AR. Site-directed mutagenesis of the Escherichia coli chromosome near oriC: identification and characterization of asnC, a regulatory element in E. coli asparagine metabolism. Nucleic Acids Res. 13(24):8797-811 (1985). [Pubmed]

Perez-Rueda E., Collado-Vides J. Common history at the origin of the position-function correlation in transcriptional regulators in archaea and bacteria. J Mol Evol. 53(3):172-9 (2001). [Pubmed]

Thaw P., Sedelnikova SE., Muranova T., Wiese S., Ayora S., Alonso JC., Brinkman AB., Akerboom J., van der Oost J., Rafferty JB. Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family. Nucleic Acids Res. 34(5):1439-1449 (2006). [Pubmed]

Yokoyama K., Ishijima SA., Clowney L., Koike H., Aramaki H., Tanaka C., Makino K., Suzuki M. Feast/famine regulatory proteins (FFRPs): Escherichia coli Lrp, AsnC and related archaeal transcription factors. FEMS Microbiol Rev. 30(1):89-108 (2006). [Pubmed]

Kolling R., Gielow A., Seufert W., Kucherer C., Messer W. AsnC, a multifunctional regulator of genes located around the replication origin of Escherichia coli, oriC. Mol Gen Genet. 212(1):99-104 (1988). [Pubmed]

Gielow A., Kucherer C., Kolling R., Messer W. Transcription in the region of the replication origin, oriC, of Escherichia coli: termination of asnC transcripts. Mol Gen Genet. 214(3):474-81 (1988). [Pubmed]

Schaefer C., Messer W. Termination of the Escherichia coli asnC transcript. The DnaA protein/dnaA box complex blocks transcribing RNA polymerase. Gene. 73(2):347-54 (1988). [Pubmed]

Poggio S., Domeinzain C., Osorio A., Camarena L. The nitrogen assimilation control (Nac) protein represses asnC and asnA transcription in Escherichia coli. FEMS Microbiol Lett. 206(2):151-6 (2002). [Pubmed]
Related annotations: PaperBLAST

Disclaimer and license

These data are available AS IS and at your own risk. The EEAD/CSIC do not give any representation or warranty nor assume any liability or responsibility for the data nor the results posted (whether as to their accuracy, completeness, quality or otherwise). Access to these data is available free of charge for ordinary use in the course of research. Downloaded data have CC-BY-NC-SA license. FootprintDB is also available at RSAT::Plants, part of the INB/ELIXIR-ES resources portfolio.