DNA Binding Motif

						

			
Accessions: SacT (DBTBS 1.0), SacY (DBTBS 1.0)

			
Names: SacT, SacY
Organisms: Bacillus subtilis
Libraries: DBTBS 1.0 1
1 Sierro N, Makita Y, de Hoon M, Nakai K. DBTBS: a database of transcriptional regulation in Bacillus subtilis containing upstream intergenic conservation information. Nucleic acids research 36:D93-6 (2008). [Pubmed]
Length: 36
Consensus: GCGGGwTTGTkACTGrkmArGCAGGCAAGACCyAAw
Weblogo: 
PSSM: P0      A      C      G      T
01      0      0      3      0      G
02      0      3      0      0      C
03      0      0      3      0      G
04      0      0      3      0      G
05      0      0      3      0      G
06      2      0      0      1      w
07      0      0      0      3      T
08      0      0      0      3      T
09      0      0      3      0      G
10      0      0      0      3      T
11      0      0      2      1      k
12      3      0      0      0      A
13      0      3      0      0      C
14      0      0      0      3      T
15      0      0      3      0      G
16      1      0      2      0      r
17      0      0      1      2      k
18      2      1      0      0      m
19      3      0      0      0      A
20      2      0      1      0      r
21      0      0      3      0      G
22      0      3      0      0      C
23      3      0      0      0      A
24      0      0      3      0      G
25      0      0      3      0      G
26      0      3      0      0      C
28      3      0      0      0      A
29      0      0      3      0      G
30      3      0      0      0      A
31      0      3      0      0      C
32      0      3      0      0      C
33      0      1      0      2      y
34      3      0      0      0      A
35      3      0      0      0      A
36      2      0      0      1      w
Type: Heterodimer
Binding TFs: SacT (PRD domain, CAT RNA binding domain)
SacY (phosphotransferase system, EIIB, Phosphotransferase system, EIIC )
Binding Sites: sacB_1 / sacB_2
sacP_1 / sacP_2
sacX_2 / sacX_3
			
Publications: Steinmetz M, Le Coq D, Aymerich S. Induction of saccharolytic enzymes by sucrose in Bacillus subtilis: evidence for two partially interchangeable regulatory pathways. Journal of bacteriology 171:1519-23 (1989). [Pubmed]


Debarbouille M, Arnaud M, Fouet A, Klier A, Rapoport G. The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators. Journal of bacteriology 172:3966-73 (1990). [Pubmed]


Arnaud M, Vary P, Zagorec M, Klier A, Debarbouille M, Postma P, Rapoport G. Regulation of the sacPA operon of Bacillus subtilis: identification of phosphotransferase system components involved in SacT activity. Journal of bacteriology 174:3161-70 (1992). [Pubmed]


Arnaud M, Débarbouillé M, Rapoport G, Saier M.H, Reizer J. In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis. The Journal of biological chemistry 271:18966-72 (1996). [Pubmed]


Tortosa P, Le Coq D. A ribonucleic antiterminator sequence (RAT) and a distant palindrome are both involved in sucrose induction of the Bacillus subtilis sacXY regulatory operon. Microbiology (Reading, England) 141 ( Pt 11):2921-7 (1995). [Pubmed]


Steinmetz M, Aymerich S. [Genetic analysis of sacR, a cis-regulator of levan-saccharase synthesis of Bacillus subtilis]. Annales de l'Institut Pasteur. Microbiologie 137A:3-14 (). [Pubmed]


Steinmetz M, Le Coq D, Aymerich S, Gonzy-Tréboul G, Gay P. The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites. Molecular & general genetics : MGG 200:220-8 (1985). [Pubmed]


Aymerich S, Gonzy-Tréboul G, Steinmetz M. 5'-noncoding region sacR is the target of all identified regulation affecting the levansucrase gene in Bacillus subtilis. Journal of bacteriology 166:993-8 (1986). [Pubmed]


Shimotsu H, Henner D.J. Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes. Journal of bacteriology 168:380-8 (1986). [Pubmed]


Klier A, Fouet A, Débarbouillé M, Kunst F, Rapoport G. Distinct control sites located upstream from the levansucrase gene of Bacillus subtilis. Molecular microbiology 1:233-41 (1987). [Pubmed]


Aymerich S, Steinmetz M. Cloning and preliminary characterization of the sacS locus from Bacillus subtilis which controls the regulation of the exoenzyme levansucrase. Molecular & general genetics : MGG 208:114-20 (1987). [Pubmed]


Aymerich S, Steinmetz M. Specificity determinants and structural features in the RNA target of the bacterial antiterminator proteins of the BglG/SacY family. Proceedings of the National Academy of Sciences of the United States of America 89:10410-4 (1992). [Pubmed]

	
			


			

				

				
				
				
				

			

			

		

	
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