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structure nameI-SCEI IN COMPLEX WITH A BOTTOM NICKED DNA SUBSTRATE
(Intron-encoded endonuclease I-SceI)
referenceQuiocho et al. 'Nucleic 36 3287 2008
sourceSaccharomyces cerevisiae
experimentX-ray (resolution=2.20, R-factor=?)
structural superfamilyHoming endonucleases;
sequence familyLAGLIDADG DNA endonuclease family;
redundant complexes1r7m_B 3c0x_A 3ool_A 3oor_A
links to other resourcesNAKB PDBSum PDIdb DNAproDB
protein sequence
interface signaturePNYRRCQEKRNHVTQWDYNNQNK

Estimated binding specificities ?

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# IC=18.184 IC/col=1.010 n_of_columns=18 specificity:   
A |  89   3   6  76   0   0   0   0   0   0  86  24   1  13   6   0   0  96
C |   0   3   8   6  96  96  96   0   0   3   4  24   3  80  86  19   3   0
G |   7   6   6   8   0   0   0   0  96   0   0  24   0   0   0   3   1   0
T |   0  84  76   6   0   0   0  96   0  93   6  24  92   3   4  74  92   0
scan!

Dendrogram of similar interfaces ?

matrix format
  
  PTNTYGRGRG----QGECKTRGNTHTVATAQC----WADTYCNT------NCQC----------------NCKT--------------   L              +------------3c0w_A    
                                                                                                 +---------10  
  ------YTLT----VTTG--------------------------RGRGWC--------------DCDC--RGHAVC------------   L   !          +------------2vs7_A    
                                                                                                 !  
  ----------------RG--------------CA--FT--NT----------------YT--LA--KG------FTWC--EATT----   L   !                         +6uw0_B    
                                                                                                 !                       +-1 
  ----------------KG--------------CA--FC--------------------YA--KG--YG--KG--FTWT--------TA   L   !                       ! +6bd0_A    
                                                                                                 !                    +--5 
  ----------------KG--------------CA--FC--------------------YG--KG--KG--KG--FTWC----TG----   L -11                    !  ! +6uwj_A    
                                                                                                 !                    !  +-3 
  ----------------RT--------------HT------------------------YG--LA--KG--RG--LTWC------RGTG   L   !                    !    +5t2n_A    
                                                                                                 !             +------7 
  ------------QG------------------CC----AC----------------------RT------KGRTVAEC--QT------   L   !             !      !    +4yit_A    
                                                                                                 !             !      !  +-2 
  --------------KT----------------CTST--------------------TG--RA--ET--RG----ATDT----TA----   L   !             !      !  ! +4lq0_A    
                                                                                                 !             !      +--6 
  --------------------------------CTVA--FT------------------------ST--DA--RGTC--KT--TG----   L   +-------------9         ! +5esp_A    
                                                                                                               !         +-4 
  ----------RG--------------------SARA--------------------TG----------RG--RGCC--------TA--   L                 !           +6bce_A    
                                                                                                               ! 
  ------------DG------------------SA--------------------IG----NA--RG--RT--DTICDG----IA----   L                 !     +---3fd2_A    
                                                                                                               +-----8 
  --------------------------------STQA--KG--------------TC----------YT--SC--VCDTRG--ST----   L                       +---3mxa_A    
                                                                                               

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updated Tue Dec 19 07:12:11 2023